Continuous Vesicle Cycling in the Synaptic Terminal of Retinal Bipolar Cells
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چکیده
منابع مشابه
Continuous Vesicle Cycling in the Synaptic Terminal of Retinal Bipolar Cells
Endocytosis and exocytosis were investigated in the synaptic terminal of retinal bipolar cells by monitoring the uptake and loss of the fluorescent dye FM1-43. Depolarization in the presence of Ca2+ stimulated a continuous cycle of exocytosis and endocytosis that was approximately balanced at rates up to 3800 vesicles per s. Vesicles became available for exocytosis within 1 min of endocytosis, ...
متن کاملCalcium influx selects the fast mode of endocytosis in the synaptic terminal of retinal bipolar cells.
To investigate the regulation of endocytosis by Ca(2+), we have made capacitance measurements in the synaptic terminal of depolarizing bipolar cells from the retina of goldfish. After a brief depolarization, all of the excess membrane was retrieved rapidly (tau approximately 1 s). But when the rise in free [Ca(2+)] was reduced by the introduction of Ca(2+) buffers [1,2-bis(2-aminophenoxy)ethane...
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Calcium-triggered exocytosis at the synapse is suppressed by addition of calcium chelators, but the effects of endogenous Ca(2+) buffers have not been tested. We find that 80% of Ca(2+) binding sites in the synaptic terminal of retinal bipolar cells were associated with mobile molecules that suppressed activation of Ca(2+)-sensitive K(+) channels with an efficiency equivalent to approximately 1...
متن کاملBulk membrane retrieval in the synaptic terminal of retinal bipolar cells.
The mechanism of bulk membrane uptake at the synapse remains poorly defined, although exocytosis of synaptic vesicles is followed by compensatory membrane retrieval into both small vesicles and large cisternas or vacuoles. We investigated bulk retrieval in the presynaptic terminal of retinal bipolar cells. Fluorescence imaging of the membrane dye FM1-43 indicated that Ca2+-triggered exocytosis ...
متن کاملThe Functional Organization of Synaptic Vesicle Pools in a Retinal Bipolar Neuron
..............................................................................................v TABLE OF CONTENTS.......................................................................................................vii LIST OF FIGURES....................................................................................viii LIST OF TABLES..............................................................
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ژورنال
عنوان ژورنال: Neuron
سال: 1996
ISSN: 0896-6273
DOI: 10.1016/s0896-6273(00)80226-3